Cell Culture

How does immune cell polarity respond to changing stimuli? Introduction Neutrophils are fast-moving immune cells that polarize and reorient in response to external signals. Using optogenetic control of PI3K, recent experiments revealed that when a localized light stimulus becomes global, cells reverse their polarity — a behaviour not predicted by classical models.

Viral Spread within the Human Airway Epithelium (HAE) Introduction The spread of viral infections within tissues depends on several factors including the spatial distribution and turnover dynamics of target cells.

Introduction Collective motion of human breast cancer cells of the cell line MDA-MB-231 was studied by Lee et al. for individual crawling cells, cell doublets, cell quadruplets and for confluent populations on a two-dimensional substrate.

Introduction Brook et al. studied the effect of distinct immune phenotypes on cellular-scale viral propagation in three mammalian kidney cell lines with different interferon pathways (IFN). Differences in the IFN response upon pathogen invasion allowed to experimentally observe, model and numerically simulate viral dynamics in cells with

Introduction In Jagiella et al. 2017 a quantitative single cell-based mathematical model for multi-cellular tumor spheroids was parametrized by Bayesian inference for different scenarios of nutrition and oxygen supply. The simulations generate of the order of a million individual cells.

Multiscale simulation of EMT by minimal signaling circuit Introduction We model epithelial-mesenchymal transition (EMT) by first assembling an ODE model for intracellular Yes-associated protein (YAP) signalling and then embedding this single cell model within individual cells in a multiscale simulation.

Simulated sheet morphology for YAP knockdown (KD) and overexpression (OE) Introduction We model epithelial-mesenchymal transition (EMT) by first assembling an ODE model for intracellular Yes-associated protein (YAP) signalling and then embedding this single cell model within individual cells in a multiscale simulation.

Multiple models in epithelial-mesenchymal transitions (EMT) Introduction We model epithelial-mesenchymal transition (EMT) by first assembling an ODE model for intracellular Yes-associated protein (YAP) signalling and then embedding this single cell model within individual cells in a multiscale simulation.

Multiscale model of epithelial-mesenchymal transition (EMT) with intracellular TGFβ signaling circuit Introduction The growth factor TGFβ regulates the snail-zeb intracellular circuit that affects EMT by controlling the levels of E and N-cadherin expression.

Introduction The formation and maintenance of three germ layers in colonies of human embryonic stem cells (ESCs) is sensitive to the geometry of the colony. In vitro experiments with micropatterns controlling colony shape have previously addressed the underlying spatio-temporal patterning in Warmflash et al.