Model ID: https://identifiers.org/morpheus/M3799 File type: Main model Software: Morpheus (open source). Download from: https://morpheus.gitlab.io Full title: Contributions of Single-cell Mechanics and Cell-cell Adhesion to Multicellular Spheroid Mechanics Authors: D. Dolgitzer, E. Parajón, D. N. Robinson, P. A. Iglesias Submitter: D. Dolgitzer Curator: D. Jahn Date: 28.09.2026 Reference: This model is the original used in the publication, up to technical updates: D. Dolgitzer, E. Parajón, D. N. Robinson, P. A. Iglesias: Contributions of single-cell mechanics and cell-cell adhesion to multicellular spheroid mechanics. bioRxiv 2026.08.04.742605, 2026. Comment: This model simulates micropipette aspiration of a spheroid made of 500 cells, used in ‘Contributions of single-cell mechanics and cell-cell adhesion to multicellular spheroid mechanics’ by Dolgitzer et al., 2026.

1

if(medium_contact==1, sqrt(cell.volume/pi), buffer + sqrt(cell.volume/pi)) if(medium_distance <= 1.5*sqrt(cell.volume/pi),0.5,1) if(stress_region_local == 1, stress_timer + 0.1, 0)

(protrusion - pipette_edge)*(protrusion > pipette_edge+1)

1 optimal optimal

(pipette_top + pipette_bot)/2

(space.y >= pipette_bot) * (space.y <= pipette_top) * (space.x >= max(pipette_edge, front)) max(pipette_edge, protrusion_top - vacuum_thickness) max(pipette_edge, protrusion_mid - vacuum_thickness) max(pipette_edge, protrusion_bot - vacuum_thickness) if(space.y <= pipette_mid, bot_x + (mid_x - bot_x) * (space.y - pipette_bot) / (pipette_mid - pipette_bot), mid_x + (top_x - mid_x) * (space.y - pipette_mid) / (pipette_top - pipette_mid) ) vacuum is applied dynamically to match the protrusion front at a thickness of 1 pixel.